P-18 Prognostic significance of microRNAs miR-497-5p and miR-548b-5p for predicting hepatitis B virus-related hepatocellular carcinoma recurrence after curative surgical resection

Hepatocellular carcinoma (HCC) is one of the most prevalent and lethal human cancers chronic hepatitis B virus (HBV) infection accounts for up to 50% total HCC cases worldwide. Although resection surgery has been available as a curative therapy HCC, high rate recurrence remains big threat patient survival. Therefore, discovery valuable biomarkers selecting patients at higher risk key goal allow timely better treatment improved prognosis. Considering that microRNAs (miRNAs) have shown play important roles in all aspects development, this study aimed identify novel miRNAs with prognostic significance predicting HBV-related after surgical resection. A cohort 68 who underwent were retrospectively recruited divided into two groups: group (n = 43) non-recurrence 25). panel 380 analyzed whose expression ratio between paired tumor (T) non-tumor (NT) tissues was significantly altered groups associated post-operative recurrence. 9 (miR-142-5p, miR-145-5p, miR-148b-3p, miR-191-5p, miR-324-5p, miR-345-5p, miR-142-5p, miR-497-5p, miR-503-5p, miR-548b-5p) showed T/NT patients. Following multivariate recurrence-free survival (RFS) analysis, only miR-497-5p (T/NT) (> 0.97 vs. ≤ 0.97), miR-548b-5p 2.54 2.54), TNM stage (III/IV I/II) validated independent (hazard 0.173, p value 0.0013; hazard 2.720, 0.0092; 2.887, 0.0031, respectively). In Kaplan-Meier RFS low (T/NT), or displayed than (p 0.0037, 0.0434, < 0.0001, Furthermore, receiver operating characteristic (ROC) analysis revealed had best performance, indicated by highest area under ROC curve (0.6902), followed (0.6502) then (0.6195). This suggests predicted